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SibEnzyme Ltd. :: Display product information: Nru I
Nru I
Catalog numberE099E100
Package, u.a.5002500
Concentration, u.a./ml5000-100005000-10000

Recognition siteTCG^CGA
Methylation sensitivityBlocked by overlapping Dam methylation (GmATC): TCGCGATC.
OriginNocardia rubra
Storage conditions10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol; Store at -20C.
Assayed onLambda DNA (dam-)
LigationAfter 5-fold overdigestion with enzyme about 20% of the DNA fragments can be ligated. In the presence of 10% PEG ligation is better.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes80oC
Optimum SE-bufferW (10 mM Tris-HCl (pH 8.5 at 25C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)

Enzyme % activity:
0 - 1010 - 2575 - 10010010 - 25

References: Comb, D.G., Schildkraut, I., Greenough, L. Unpublished observations.