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SibEnzyme Ltd. :: Display product information: MspR9 I
MspR9 I
Catalog numberE175E176
Package, u.a.5002500
Concentration, u.a./ml10000-3000010000-30000

Recognition siteCC^NGG
Methylation sensitivityBlocked by overlapping Dcm methylation(CmCWGG): CCAGG and CCTGG.
OriginMicrococcus species R9
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoetyanol; 100 ug/ml BSA; and 50% glycerol; Store at -20C.
Assayed onLambda DNA (dcm-)
LigationAfter 2-fold MspR9 I overdigestion none of the DNA fragments can be ligated.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 30 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes80oC
Optimum SE-bufferO (50 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)

Enzyme % activity:
50 - 7550 - 7510050 - 7575 - 100

References: Repin, V.E., Andreeva, I.S., Kileva, E.V., Shevchenko, A.V., Abdurashitov, M.A., Repin, M.V., Degtyarev, S.Kh. Prikl. Biokhim. Mikrobiol. 33: 284-286 (1997).