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SibEnzyme Ltd. :: Display product information: MspA1 I
MspA1 I
Catalog numberE191E192
Package, u.a.5002500
Concentration, u.a./ml2000-100002000-10000

Recognition siteCMG^CKG
Methylation sensitivitynot tested
OriginMoraxella species A1
Storage conditions20 mM Tris-HCl (pH 7.6); 300 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 10 mM MgCl2, 200 ug/ml BSA, 50% glycerol. Store at -20C. Store at -70ºC is recommended for period longer than 30 days.
Assayed onLambda DNA
LigationAfter 5-fold overdigestion with enzyme 60% of the DNA fragments can be ligated and recut. In the presence of 10% PEG ligation is better
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)

Enzyme % activity:
10 - 2575 - 10010 - 2525 - 50100

References: Degtyarev, S.K. Unpublished observations.