Name | Msp I | Catalog number | E091 | E092 | Package, u.a. | 1000 | 5000 | Concentration, u.a./ml | 10000-30000 | 10000-30000 |
Recognition site | C^CGG GGC^C | Methylation sensitivity | not tested | Origin | Moraxella species | Storage conditions | 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol; Store at -20°C. | Assayed on | Lambda DNA | Ligation | After 10-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut. | Non-specific activity | No nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C. | Optimum temperature | 37 oC | Inactivation 20 minutes | 65oC | Optimum SE-buffer | B (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.) |
Enzyme % activity:
B | G | O | W | Y | 100 | 75 - 100 | 50 - 75 | 75 - 100 | 75 - 100 |
References: Waalwijk, C., Flavell, R.A., Nucl. Acids Res.5: 3231-3236 (1978).
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