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SibEnzyme Ltd. :: Display product information: Msp I
Name
Msp I
Catalog numberE091E092
Package, u.a.10005000
Concentration, u.a./ml10000-3000010000-30000

Recognition siteC^CGG
GGC^C
Methylation sensitivitynot tested
OriginMoraxella species
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol; Store at -20C.
Assayed onLambda DNA
LigationAfter 10-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferB (10 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 1 mM DTT.)

Enzyme % activity:
BGOWY
10075 - 10050 - 7575 - 10075 - 100

References: Waalwijk, C., Flavell, R.A., Nucl. Acids Res.5: 3231-3236 (1978).