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SibEnzyme Ltd. :: Display product information: MroN I
Name
MroN I
Catalog numberE087E088
Package, u.a.5002500
Concentration, u.a./ml2000-100002000-10000

Recognition siteG^CCGGC
CGGCC^G
Methylation sensitivitynot tested
OriginMicrococcus roseus N0
Storage conditions10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol; Store at -20C.
Assayed onAdenovirus-2 DNA
LigationAfter 5-fold overdigestion with enzyme more than 90% of the Ad-2 DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Ad2 DNA with 5 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes80oC
Optimum SE-bufferB (10 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 1 mM DTT.)

Enzyme % activity:
BGOWY
10050 - 7510 - 250 - 1010 - 25

References: Dedkov, V.S., Belichenko, O.A., Prikhod'ko, E.A., Abdurashitov, M.A., Degtyarev, S.K. Unpublished observations (1994).