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SibEnzyme Ltd. :: Display product information: Mly113 I
Name
Mly113 I
Catalog numberE189E190
Package, u.a.2001000
Concentration, u.a./ml3000-50003000-5000

Recognition siteGG^CGCC
CCGC^GG
Methylation sensitivitynot tested
OriginMicrococcus lylae 113
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol; Store at -20C.
Assayed onT7 DNA
LigationAfter 3-fold overdigestion with enzyme more than 80% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of T7 DNA with 10 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferB (10 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 1 mM DTT.)

Enzyme % activity:
BGOWY
10025 - 5010 - 2510 - 2550 - 75

Note: High enzyme concentration may result in star activity.
References: Dedkov, V.S., Zernov, Y.P., Rechkunova, N.I., Degtyarev, S.Kh. Mol.Genet.Mikrobiol.Virusol. 12: 17-18 (1990).