Go to sibenzyme.com
SibEnzyme Ltd. :: Display product information: Mlu I
Name
Mlu I
Catalog numberE085E086
Package, u.a.10005000
Concentration, u.a./ml10000-3000010000-30000

Recognition siteA^CGCGT
TGCGC^A
Methylation sensitivitynot tested
OriginMicrococcus luteus
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol; Store at -20C.
Assayed onLambda DNA
LigationAfter 10-fold overdigestion with enzyme 95% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferO (50 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)

Enzyme % activity:
BGOWY
0 - 1010 - 2510025 - 5010 - 25

References: Sugisaki, H., Kanazawa, S. Gene 16: 73-78 (1981).