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SibEnzyme Ltd. :: Display product information: Msp20 I
Msp20 I
Catalog numberE301E302
Package, u.a.100500
Concentration, u.a./ml1000-30001000-3000

Recognition siteTGG^CCA
Methylation sensitivityCleaved of DNA is impaired by overlapping Dcm methylation(CmCWGG): TGGCCAGG.
OriginMicrococcus species 20
Storage conditions10 mM Tris-HCl (pH 7.5); 200 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol; Store at -20C.
Assayed onLanbda DNA (dcm-)
LigationAfter 2-fold overdigestion with enzyme 80% of the DNA fragments can be ligated and recut. More complete ligation can be reached by using 10% PEG
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 2 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferW (10 mM Tris-HCl (pH 8.5 at 25C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) + BSA

Enzyme % activity:
50 - 7550 - 7525 - 5010050 - 75

Note: To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 ug/ml.
Do not use BSA for long incubation.
References: Chernov, A.P., Belichenko, O.A., Rechkunova, N.I., Andreeva, I.S., Repin, V.E., Degtyarev, S.K. Russian Patent Office (1993). SU 1806191 A3