|Catalog number||E187||E188 |
|Package, u.a.||200||1000 |
|Concentration, u.a./ml||1000-5000||1000-5000 |
|Methylation sensitivity||Not blocked by overlapping Dam methylation (GmATC): GATC.|
Blocked by overlapping CG methylation: CGATmCG.
Cleaved of DNA is impaired by overlapping CG methylation: GATmCG.
|Origin||Kurthia zopfii 9|
|Storage conditions||10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; and 50% glycerol; Store at -20°C.|
|Assayed on||Lambda DNA|
|Ligation||After 5-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.|
|Non-specific activity||No nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 2 u.a. of enzyme for 16 hours at 37°C.|
|Optimum temperature||37 oC|
|Inactivation 20 minutes||65oC|
|Optimum SE-buffer||G (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.)|
Enzyme % activity:
|50 - 75||100||50 - 75||50 - 75||50 - 75|
References: Degtyarev, S.Kh., Rechkunova, N.I., Grinev, A.A., Dedkov, V,S., Izv.Sib.Otd.Akad.Nauk SSSR 15: 25-26 (1989).