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SibEnzyme Ltd. :: Display product information: Kzo9 I
Kzo9 I
Catalog numberE187E188
Package, u.a.2001000
Concentration, u.a./ml1000-50001000-5000

Recognition site^GATC
Methylation sensitivityNot blocked by overlapping Dam methylation (GmATC): GATC.
Blocked by overlapping CG methylation: CGATmCG.
Cleaved of DNA is impaired by overlapping CG methylation: GATmCG.
OriginKurthia zopfii 9
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; and 50% glycerol; Store at -20C.
Assayed onLambda DNA
LigationAfter 5-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 2 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferG (10 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.)

Enzyme % activity:
50 - 7510050 - 7550 - 7550 - 75

References: Degtyarev, S.Kh., Rechkunova, N.I., Grinev, A.A., Dedkov, V,S., Izv.Sib.Otd.Akad.Nauk SSSR 15: 25-26 (1989).