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SibEnzyme Ltd. :: Display product information: AccBS I
Catalog numberE007E008
Package, u.a.10005000
Concentration, u.a./ml5000-200005000-20000

Recognition siteGAG^CGG
Methylation sensitivitynot tested
OriginAcinetobacter calcoaceticus BS
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol; Store at -20C.
Assayed onLambda DNA
LigationAfter 5-fold overdigestion with enzyme 90% of DNA fragments can be ligated. Of these 50% can be recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)

Enzyme % activity:
75 - 10075 - 10025 - 5025 - 50100


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