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SibEnzyme Ltd. :: Display product information: Ksp22 I
Ksp22 I
Catalog numberE081E082
Package, u.a.10005000
Concentration, u.a./ml10000-3000010000-30000

Recognition siteT^GATCA
Methylation sensitivityBlocked by Dam methylation (GmATC): TGATCA
OriginKurthia species 22
Storage conditions10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA, 50% glycerol; Store at -20C.
Assayed onLambda DNA (dam-)
LigationAfter 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-buffer2K (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 200 mM KCl; 1 mM DTT.) + BSA

Enzyme % activity:
50 - 7575 - 10050 - 7550 - 7525 - 50

Note: To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 ug/ml.
Do not use BSA for long incubation.
References: Dedkov, V,S., Degtyarev, S.Kh., Unpublished observations.