|Catalog number||E081||E082 |
|Package, u.a.||1000||5000 |
|Concentration, u.a./ml||10000-30000||10000-30000 |
|Methylation sensitivity||Blocked by Dam methylation (GmATC): TGATCA|
|Origin||Kurthia species 22|
|Storage conditions||10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA, 50% glycerol; Store at -20°C.|
|Assayed on||Lambda DNA (dam-)|
|Ligation||After 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.|
|Non-specific activity||No nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.|
|Optimum temperature||37 oC|
|Inactivation 20 minutes||65oC|
|Optimum SE-buffer||2K (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 200 mM KCl; 1 mM DTT.) + BSA|
Enzyme % activity:
|50 - 75||75 - 100||50 - 75||50 - 75||25 - 50|
Note: To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 ug/ml.
Do not use BSA for long incubation.
References: Dedkov, V,S., Degtyarev, S.Kh., Unpublished observations.