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SibEnzyme Ltd. :: Display product information: Kpn I
Kpn I
Catalog numberE079E080E079XE080X
Package, u.a.200010000200010000
Concentration, u.a./ml20000200004000040000

Recognition siteGGTAC^C
Methylation sensitivityNot blocked by overlapping Dcm methylation (CmCWGG): GGTACCWGG
OriginIsolated from an E.coli strain that carries the cloned Kpn I gene from Klebsiella pneumonia
Storage conditions10 mM Tris-HCl(pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20C.
Assayed onLambda DNA
LigationAfter 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes80oC
Optimum SE-bufferB (10 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 1 mM DTT.) + BSA

Enzyme % activity:
10025 - 5025 - 5025 - 5075 - 100

Note: High enzyme concentration may result in star activity.
Long incubation with BSA is not recmmenden due to star activity.
To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 ug/ml.
References: Smith, D.I., Blattner, F.R., Davies, J. Nucleic Acids Res. 3: 343-353 (1976).