|Catalog number||E079||E080||E079X||E080X |
|Package, u.a.||2000||10000||2000||10000 |
|Concentration, u.a./ml||20000||20000||40000||40000 |
|Methylation sensitivity||Not blocked by overlapping Dcm methylation (CmCWGG): GGTACCWGG|
|Origin||Isolated from an E.coli strain that carries the cloned Kpn I gene from Klebsiella pneumonia|
|Storage conditions||10 mM Tris-HCl(pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.|
|Assayed on||Lambda DNA|
|Ligation||After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.|
|Non-specific activity||No nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.|
|Optimum temperature||37 oC|
|Inactivation 20 minutes||80oC|
|Optimum SE-buffer||B (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.) + BSA|
Enzyme % activity:
|100||25 - 50||25 - 50||25 - 50||75 - 100|
Note: High enzyme concentration may result in star activity.
Long incubation with BSA is not recmmenden due to star activity.
To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 ug/ml.
References: Smith, D.I., Blattner, F.R., Davies, J. Nucleic Acids Res. 3: 343-353 (1976).