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SibEnzyme Ltd. :: Display product information: HspA I
HspA I
Catalog numberE069E070
Package, u.a.10005000
Concentration, u.a./ml2000020000

Recognition siteG^CGC
Methylation sensitivityBlocked by CG methylation 5'-G(5mC)GC-3'/3'-CG(5mC)G-5'.
Not blocked by methylation 5'-GCG(5mC)-3'/3'-CGCG-5'.
Cut hemimethylated site: 5'- G(5mC)GC-3'/3'-CGCG-5
OriginIsolated from an E.coli strain that carries the cloned HspA I gene from Haemophilus species A1
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20C.
Assayed onLambda DNA
LigationAfter 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes80oC
Optimum SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)

Enzyme % activity:
50 - 7550 - 7525 - 5025 - 50100

References: Rechkunova, N.I., Prikhod'ko, E.A., Shevchenko, A.V., Degtyarev, S.K. Unpublished observations (1994).