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SibEnzyme Ltd. :: Display product information: Hpa II
Name
Hpa II
Catalog numberE161E162
Package, u.a.5002500
Concentration, u.a./ml1000010000

Recognition siteC^CGG
GGC^C
Methylation sensitivityBlocked by CG methylation.
OriginE.coli strain, that carries the cloned gene Hpa II from Haemophilus parainfluenzae
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 100 μg/ml BSA; 50% glycerol; Store at -20C.
Assayed onLambda DNA
LigationAfter 10-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes80oC
Optimum SE-bufferB (10 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 1 mM DTT.)

Enzyme % activity:
BGOWY
10050 - 7510 - 2525 - 5050 - 75

References: Sharp, P.A., Sugden, B., Sambrook, J. Biochemistry 12: 3055-3063 (1973).