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SibEnzyme Ltd. :: Display product information: Hinf I
Hinf I
Catalog numberE075E076E075XE076X
Package, u.a.1000500010005000
Concentration, u.a./ml20000200004000040000

Recognition siteG^ANTC
Methylation sensitivitynot tested
OriginE.coli strain, that carries the cloned gene HinfI from Haemophilus influenzae
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol; Store at -20C.
Assayed onLambda DNA
LigationAfter 20-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes80oC
Optimum SE-bufferO (50 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)

Enzyme % activity:
25 - 5075 - 10010075 - 10075 - 100

References: Hutchison, C.A., Barrell, B.G. Unpublished observations. (1992)