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SibEnzyme Ltd. :: Display product information: Hind III
Hind III
Catalog numberE073E074E073XE074X
Package, u.a.500025000500025000
Concentration, u.a./ml2000020000100000100000

Recognition siteA^AGCTT
Methylation sensitivitynot tested
OriginE.coli strain, that carries the cloned gene Hind III from Haemophilus influenzae Rd
Storage conditions10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20C.
Assayed onLambda DNA
LigationAfter 50-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes80oC
Optimum SE-bufferW (10 mM Tris-HCl (pH 8.5 at 25C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) + BSA

Enzyme % activity:
10 - 2525 - 500 - 101000 - 10

Note: To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 ug/ml.
Do not use BSA for long incubation.
References: Old, R., Murray, K., Roizes, G. J. Mol. Biol. 92: 331-339 (1975).