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SibEnzyme Ltd. :: Display product information: Hae III
Catalog numberE067E068E068X
Package, u.a.20001000010000
Concentration, u.a./ml100001000050000

Recognition siteGG^CC
Methylation sensitivitynot tested
OriginE.coli strain, that carries the cloned Hae III gene from Haemophilus aegyptius
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol; Store at -20C.
Assayed onLambda DNA
LigationAfter 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes80oC
Optimum SE-bufferG (10 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.)

Enzyme % activity:
75 - 10010025 - 5050 - 7550 - 75

References: Middleton, J.H., Edgell, M.H., Hutchison, C.A. J. Virol. 10:42-50 (1972).