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Name Fsp4H I Catalog number E095 E096 Package, u.a. 100 500 Concentration, u.a./ml 1000-5000 1000-5000 Recognition site GC^NGC CGN^CG Methylation sensitivity not tested Origin Flavobacterium species 4H Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol; Store at -20°C. Assayed on Lambda DNA Ligation After 2-fold overdigestion with enzyme about 5% of the DNA fragments can be ligated and recut. Non-specific activity No nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 4 u.a. of enzyme for 16 hours at 37°C. Optimum temperature 37 oC Inactivation 20 minutes 65oC Optimum SE-buffer Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) Enzyme % activity: B G O W Y 50 - 75 75 - 100 10 - 25 25 - 50 100 References: Dedkov, V.S., Kileva, E.V., Shevchenko, A.V., Degtyarev, S.K. Unpublished observations (1995). Chmuzh E.V., Kashirina Yu.G., Tomilova Yu.E., Chernukhin V.A., Okhapkina S.S., Gonchar D. A., Dedkov V.S., Abdurashitov M. A., Degtyarev S. Kh. Molecular Biology, V.41, No 1, pp 43-50 (2007) (In Russian)