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SibEnzyme Ltd. :: Display product information: FriO I
Name
FriO I
Catalog numberE157E158
Package, u.a.10005000
Concentration, u.a./ml10000-4000010000-40000

Recognition siteGRGCY^C
C^YCGRG
Methylation sensitivitynot tested
OriginFlavobacterium rigense O
Storage conditions10 mM Tris-HCl (pH 7.5); 200 mM KCl; 0.1 mM EDTA; 200 ug/ml BSA; 7 mM 2-mercaptoethanol; 50% glycerol; Store at -20C.
Assayed onLambda DNA
LigationAfter 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) + BSA

Enzyme % activity:
BGOWY
75 - 10075 - 10010 - 250 - 10100

Note: To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 ug/ml.
Do not use BSA for long incubation.
References: Belichenko, O.A., Prikhod'ko, E.A., Abdurashitov, M.A., Degtyarev, S.K. Unpublished observations (1994).