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SibEnzyme Ltd. :: Display product information: Fok I
Fok I
Catalog numberE247E248
Package, u.a.100500
Concentration, u.a./ml1000-20001000-2000

Recognition siteGGATG(N)9^
Methylation sensitivitynot tested
OriginE.coli strain that carries the cloned Fok I gene from Flavobacterium okeanokoites
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20C.
Assayed onLambda DNA
LigationAfter 2-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 1 u.a. of enzyme for 16 hours at 37C
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)

Enzyme % activity:
50 - 7550 - 7525 - 5025 - 50100

Note: Overdigestions of > 5 units of Fok I per 1ug of DNA and incubation times > 2 hours are not recommended.
References: Sugisaki, H., Kanazawa, S., Gene 16: 73-78 (1981).