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SibEnzyme Ltd. :: Display product information: AccB7 I
Name
AccB7 I
Catalog numberE179E180
Package, u.a.2001000
Concentration, u.a./ml50005000

Recognition siteCCANNNN^NTGG
GGTN^NNNNACC
Methylation sensitivityBlocked by overlapping Dcm methylation (CmCWGG): CCANNNCCTGG or CCAGGNNNTGG.
OriginAcinetobacter calcoaceticus B7
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20C.
Assayed onLambda DNA (dcm-)
LigationAfter 5-fold overdigestion with enzyme 95% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferG (10 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.)

Enzyme % activity:
BGOWY
10 - 2510025 - 5050 - 7550 - 75

Note: High enzyme concentration may result in star activity.
References: Dedkov, V.S., Repin, V.E., Rechkunova, N.I., Degtyarev, S.Kh., Verhosina, V.A.,Vinogradova, T.P, Izv. Sib. Otd. Akad. Nauk SSSR 1: 35-37 (1990).