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SibEnzyme Ltd. :: Display product information: Fbl I
Fbl I
Catalog numberE271E272
Package, u.a.100500
Concentration, u.a./ml1000-20001000-2000

Recognition siteGT^MKAC
Methylation sensitivitynot tested
OriginE.coli strain that carries the cloned Fbl I gene from Flavobacterium balustinum
Storage conditions10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA, and 50% glycerol; Store at -20C.
Assayed onLambda DNA
LigationAfter 2-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 2 u.a. of enzyme for 16 hours at 55C.
Optimum temperature55 oC
Inactivation 20 minutes80oC
Optimum SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)

Enzyme % activity:
50 - 7575 - 1000 - 1050 - 75100

References: Degtyarev, S.K. Unpublished observations.