|Catalog number||E209||E210 |
|Package, u.a.||50||250 |
|Concentration, u.a./ml||2000||2000 |
|Methylation sensitivity||Blocked by CG methylation.|
|Origin||E.coli strain that carries the cloned Fau I gene from Flavobacterium aquatili|
|Storage conditions||10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycero; Store at -20°C.|
|Assayed on||Lambda DNA|
|Ligation||After 2-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and of these 95% can be recut.|
|Non-specific activity||No nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 4 u.a. of enzyme for 16 hours at 55°C|
|Optimum temperature||55 oC|
|Inactivation 20 minutes||65oC|
|Optimum SE-buffer||B (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.)|
Enzyme % activity:
|100||25 - 50||0 - 10||0 - 10||50 - 75|
Note: The minimum number of units that resulted in complete digestion of 1 ug of substrate DNA in 16 hours is 0,5.
References: Degtyarev, S.Kh., Kolyhalov, A.A., Rechkunova,N.I., Dedkov, V.S. Bioorg. Khim. 15: 130-132 (1989).