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SibEnzyme Ltd. :: Display product information: Fau I
Fau I
Catalog numberE209E210
Package, u.a.50250
Concentration, u.a./ml20002000

Recognition siteCCCGC(N)4^
Methylation sensitivityBlocked by CG methylation.
OriginE.coli strain that carries the cloned Fau I gene from Flavobacterium aquatili
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycero; Store at -20C.
Assayed onLambda DNA
LigationAfter 2-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and of these 95% can be recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 4 u.a. of enzyme for 16 hours at 55C
Optimum temperature55 oC
Inactivation 20 minutes65oC
Optimum SE-bufferB (10 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 1 mM DTT.)

Enzyme % activity:
10025 - 500 - 100 - 1050 - 75

Note: The minimum number of units that resulted in complete digestion of 1 ug of substrate DNA in 16 hours is 0,5.
References: Degtyarev, S.Kh., Kolyhalov, A.A., Rechkunova,N.I., Dedkov, V.S. Bioorg. Khim. 15: 130-132 (1989).