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SibEnzyme Ltd. :: Display product information: Erh I
Erh I
Catalog numberE061E062
Package, u.a.10005000
Concentration, u.a./ml10000-5000010000-50000

Recognition siteC^CWWGG
Methylation sensitivitynot tested
OriginErwinia rhapontici
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 200 ug/ml BSA; 7 mM 2-mercaptoethanol; 50% glycerol; Store at -20C.
Assayed onLambda DNA
LigationAfter 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 50 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-buffer2W (20 mM Tris-HCl (pH 8,5 at 25°C); 10 mM MgCl2;; 200 mM NaCl; 1 mM DTT) + BSA

Enzyme % activity:
10 - 2525 - 5075 - 10075 - 10010 - 25

Note: To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 ug/ml.
Do not use BSA for long incubation.
References: Zhilkina, O.A., Rechkunova, N.I., Semenchenko, G.V., Degtyarev, S.K. Unpublished observations (1994).