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SibEnzyme Ltd. :: Display product information: EcoR V
Name
EcoR V
Catalog numberE059E060
Package, u.a.200010000
Concentration, u.a./ml2000020000

Recognition siteGAT^ATC
CTA^TAG
Methylation sensitivitynot tested
OriginE.coli strain, that carries the cloned gene EcoRV from Escherichia coli
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20C.
Assayed onLambda DNA
LigationAfter 20-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 20 u.a. of enzyme fro 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes80oC
Optimum SE-bufferW (10 mM Tris-HCl (pH 8.5 at 25C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) + BSA

Enzyme % activity:
BGOWY
0 - 1025 - 5050 - 7510025 - 50

Note: High enzyme concentration results in star activity.
Do not use BSA for long incubation
To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 ug/ml.
References: Kholmina, .V., Rebentish, B.A., Skoblov, Yu.S., et.al. Dokl.Akad. Nauk SSSR 253: 495-497 (1980).