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SibEnzyme Ltd. :: Display product information: EcoR I
Name
EcoR I
Catalog numberE057E058E057XE058X
Package, u.a.500025000500025000
Concentration, u.a./ml20000200005000050000

Recognition siteG^AATTC
CTTAA^G
Methylation sensitivitynot tested
OriginE.coli strain that carries the cloned EcoR I gene from Escherichia coli
Storage conditions10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerolerol; Store at -20C.
Assayed onLambda DNA
LigationAfter 40-fold overdigestion with enzyme about 95% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferSE-buffer EcoRI (100 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.) + BSA

Enzyme % activity:
BGOWY
50 - 7575 - 10075 - 10075 - 10050 - 75

Note: High enzyme concentration and using of nonoptimal buffer may result in star activity.
Do not use BSA for long incubation.
To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 ug/ml.
References: Albertsen, H.M., Le Paslier, D., Abderrahim, H., Dausset, J., Cann, H., Cohen, D. Nucleiv Acids Res. 17: 808 (1989).