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SibEnzyme Ltd. :: Display product information: DseD I
Name
DseD I
Catalog numberE241E242
Package, u.a.5002500
Concentration, u.a./ml10000-3000010000-30000

Recognition siteGACNNNN^NNGTC
CTGNN^NNNNCAG
Methylation sensitivitynot tested
OriginDeinococcus species D2
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 200 ug/ml BSA; 7 mM 2-mercaptoethanol; 50% glycerol; Store at -20C.
Assayed onLambda DNA
LigationAfter 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37C
Optimum temperature37 oC
Inactivation 20 minutes80oC
Optimum SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) + BSA

Enzyme % activity:
BGOWY
75 - 10075 - 10025 - 5050 - 75100

Note: To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 ug/ml
Do not use BSA for long incubation.
References: Abdurashitov, M.A., Kileva, E.V., Dedkov, V.S., Degtyarev, S.K. Unpublished observations (1996).