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SibEnzyme Ltd. :: Display product information: Dra I
Name
Dra I
Catalog numberE055E056
Package, u.a.10005000
Concentration, u.a./ml10000-3000010000-30000

Recognition siteTTT^AAA
AAA^TTT
Methylation sensitivitynot tested
OriginDeinococcus radiophilus
Storage conditions10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 ug/ml BSA, 50% glycerol; Store at -20C.
Assayed onLambda DNA
LigationAfter 10-fold overdigestion with enzyme about 70% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 30 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferG (10 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.) + BSA

Enzyme % activity:
BGOWY
75 - 10010025 - 5075 - 10075 - 100

Note: To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 ug/ml.
Do not use BSA for long incubation.
References: Purvis, I.J., Moseley, B.E.B., Nucleic Acids Res. 11:5467-5474 (1983).