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SibEnzyme Ltd. :: Display product information: CciN I
CciN I
Catalog numberE203E204
Package, u.a.2001000
Concentration, u.a./ml2000-50002000-5000

Recognition siteGC^GGCCGC
Methylation sensitivityBlocked by CG methylation.
OriginCurtobacterium citreus N
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol. Store at -20C.
Assayed onAdenovirus-2 DNA
LigationAfter 5-fold overdigestion with enzyme about 95% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of DNA Ad-2 with 10 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)

Enzyme % activity:
25 - 5050 - 7575 - 10075 - 100100

Note: High enzyme concentration may result in star activity.
References: Verchozina, V.A., Degtyarev, S.Kh. Gene 157: 99-100 (1995).