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Name AccB1 I Catalog number E163 E164 Package, u.a. 500 2500 Concentration, u.a./ml 5000-10000 5000-10000 Recognition site G^GYRCC CCRYG^G Methylation sensitivity not tested Origin Acinetobacter calcoaceticus B1 Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol; Store at -20°C. Assayed on Lambda DNA Ligation After 10-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut. Non-specific activity No nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C. Optimum temperature 37 oC Inactivation 20 minutes 65oC Optimum SE-buffer K (10 mM Tris-HCl (pH 7.6 at 250C); 10 mM MgCl2; 100 mM KCl; 1 mM DTT.) + BSA Enzyme % activity: B G O W Y 50 - 75 10 - 25 10 - 25 75 - 100 50 - 75 Note: High enzyme concentration may result in star activity To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 ug/ml. Do not use BSA for long incubation. References: Degtyarev, S.Kh., Dedkov, V.S., Belichenko, O.A., Verchosina, V.A., Kusner, Y.S., Unpublished observations.