Go to sibenzyme.com
SibEnzyme Ltd. :: Display product information: AccB1 I
Name
AccB1 I
Catalog numberE163E164
Package, u.a.5002500
Concentration, u.a./ml5000-100005000-10000

Recognition siteG^GYRCC
CCRYG^G
Methylation sensitivitynot tested
OriginAcinetobacter calcoaceticus B1
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol; Store at -20C.
Assayed onLambda DNA
LigationAfter 10-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferK (10 mM Tris-HCl (pH 7.6 at 250C); 10 mM MgCl2; 100 mM KCl; 1 mM DTT.) + BSA

Enzyme % activity:
BGOWY
50 - 7510 - 2510 - 2575 - 10050 - 75

Note: High enzyme concentration may result in star activity
To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 ug/ml.
Do not use BSA for long incubation.
References: Degtyarev, S.Kh., Dedkov, V.S., Belichenko, O.A., Verchosina, V.A., Kusner, Y.S., Unpublished observations.