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SibEnzyme Ltd. :: Display product information: Btr I
Name
Btr I
Catalog numberE277E278
Package, u.a.100500
Concentration, u.a./ml2000-50002000-5000

Recognition siteCAC^GTC
GTG^CAG
Methylation sensitivitynot tested
OriginBacillus stearothermophilus SE-U62
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; and 50% glycerol; Store at -20C.
Assayed onLambda DNA
LigationAfter 3-fold overdigestion with enzyme 80% of DNA fragments can be ligated. Of these 90% can be recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 5 u.a. of enzyme for 16 hours at 60C.
Optimum temperature60 oC
Inactivation 20 minutes80oC
Optimum SE-bufferO (50 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) + BSA

Enzyme % activity:
BGOWY
75 - 10075 - 10010075 - 10075 - 100

Note: High enzyme concentration may result in star activity.
To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 ug/ml.
Do not use BSA for long incubation.
References: Degtyarev, S.Kh., Belichenko, O.A., Lebedeva, N.A., Dedkov, V.S., Abdurashitov, M.A. Nucleic Acids Res. 28: p56 (2000).