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SibEnzyme Ltd. :: Display product information: BsuR I
Name
BsuR I
Catalog numberE053E054
Package, u.a.10005000
Concentration, u.a./ml2000020000

Recognition siteGG^CC
CC^GG
Methylation sensitivitynot tested
OriginBacillus subtilis R
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 200 ug/ml BSA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20C.
Assayed onLambda DNA
LigationAfter 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes80oC
Optimum SE-bufferG (10 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.)

Enzyme % activity:
BGOWY
75 - 10010025 - 5050 - 7550 - 75

References: Bron, S., Murray, K., Trautner, T.A. Mol. Gen. Genet. 143: 13-23 (1975).