Go to sibenzyme.com
SibEnzyme Ltd. :: Display product information: Pce I
Name
Pce I
Catalog numberE105E106
Package, u.a.10005000
Concentration, u.a./ml10000-2000010000-20000

Recognition siteAGG^CCT
TCC^GGA
Methylation sensitivitynot tested
OriginPlanococcus citreus 55
Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 ug/ml BSA; 50% glycerol; Store at -20C.
Assayed onLambda DNA
LigationAfter 10-fold overdigestion with enzyme 70% of the DNA fragments can be ligated and recut
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 20 u.a. of enzyme for 16 hours at 50C.
Optimum temperature50 oC
Inactivation 20 minutes80oC
Optimum SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)

Enzyme % activity:
BGOWY
75 - 10075 - 10050 - 7525 - 50100

Note: At 37C activity is 50% from maximum
References: Gonchar, D.A., Shinkarenko, N.M., Dedkov, V.S., Popichenko, D.V., Degtyarev, S.K. Unpublished observations (2001).