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SibEnzyme Ltd. :: Display product information: FaiI
Name
FaiI
Catalog numberE551E552
Package, u.a.50250
Concentration, u.a./ml10001000

Recognition siteYA^TR
RT^AY
OriginFlavobacterium aquatile B15
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20C.
Assayed onDouble-stranded oligonucleotide
5- CGAGTTCA^TAGCTGGGCCCAAC -3
3- GCTCAAGT^ATCGACCCGGGTTG -5
LigationAfter 3-fold overdigestion with enzyme about 90% of the pUC19 DNA fragments can be ligated with DNA Ligase and recut.
Non-specific activityFai I overnight digestion greatly enhances the star activity. DNA can be digested to small oligos under "star" conditions.
Optimum temperature50 oC
UnitOne unit is defined as the amount of enzyme required to cleave 1 pmol of the double-stranded oligonucleotide of the above indicated structure in 1 hour at 50C in a total reaction volume of 20 μl.
Inactivation 20 minutes80oC
Optimum SE-bufferB (10 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 1 mM DTT.)

Enzyme % activity:
BGOWY
10050 - 7510 - 2525 - 5025 - 50