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SibEnzyme Ltd. :: Display product information: BstX2 I
Name
BstX2 I
Catalog numberE229E230
Package, u.a.5002500
Concentration, u.a./ml1000010000

Recognition siteR^GATCY
YCTAG^R
Methylation sensitivityNot blocked by Dam-methylation (GmATC): RGATCY
OriginIsolated from E.coli strain that carries the cloned BstX2I gene from Bacillus stearothermophilus X2
Storage conditions10 mM Tris-HCl (pH 7.6); 100 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Store at -20C.
Assayed onLambda DNA
LigationAfter 10-fold overdigestion with enzyme 95% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 10 u.a. of enzyme for 16 hours at 60C.
Optimum temperature60 oC
Inactivation 20 minutes80oC
Optimum SE-bufferG (10 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.)

Enzyme % activity:
BGOWY
75 - 1001000 - 1010 - 2525 - 50

References: Belichenko, O.A., Shevchenko, A.V., Dedkov, V.S., Abdurashitov, M.A., Degtyarev, S.K. Unpublished observations (1995).