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SibEnzyme Ltd. :: Display product information: RsaN I
RsaN I
Catalog numberE555E556
Package, u.a.2001000
Concentration, u.a./ml50005000

Recognition siteG^TAC
Methylation sensitivitynot tested
OriginRhodopseudomonas sphaeroides N
Storage conditions10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA, 50% glycerol. Store at -20C.
Assayed onLambda DNA
LigationAfter 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated with T4 DNA Ligase and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes80oC
Optimum SE-bufferB (10 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 1 mM DTT.)

Enzyme % activity:
10075 - 10050 - 7550 - 7575 - 100