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Name RsaN I Catalog number E555 E556 Package, u.a. 200 1000 Concentration, u.a./ml 5000 5000 Recognition site G^TAC CAT^G Methylation sensitivity not tested Origin Rhodopseudomonas sphaeroides N Storage conditions 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA, 50% glycerol. Store at -20°C. Assayed on Lambda DNA Ligation After 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated with T4 DNA Ligase and recut. Non-specific activity No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C. Optimum temperature 37 oC Inactivation 20 minutes 80oC Optimum SE-buffer B (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.) Enzyme % activity: B G O W Y 100 75 - 100 50 - 75 50 - 75 75 - 100