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SibEnzyme Ltd. :: Display product information: BstSL I
Name
BstSL I
Catalog numberE561E562
Package, u.a.2001000
Concentration, u.a./ml1000010000

Recognition siteGKGCM^C
C^MCGKG
Methylation sensitivityNot blocked by overlapping dcm-methylation (CmCWGG): GKGCCCWGG
Blocked by GKG(5mC)MC methylation
OriginBacillus stearothermophilus S
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; and 50% glycerol; Store at -20C.
Assayed onLambda DNA
LigationAfter 5-fold overdigestion with enzyme 80% of the DNA fragments can be ligated. Of these, 95% can be recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 10 u.a. of enzyme for 16 hours at 55C.
Optimum temperature55 oC
Inactivation 20 minutes65oC
Optimum SE-bufferG (10 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.) + BSA

Enzyme % activity:
BGOWY
50 - 7510050 - 7575 - 10075 - 100

Note: To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 ug/ml.
Do not use BSA for long incubation.