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SibEnzyme Ltd. :: Display product information: BspFN I
Name
BspFN I
Catalog numberE557E558
Package, u.a.5002500
Concentration, u.a./ml50005000

Recognition siteCG^CG
GC^GC
Methylation sensitivityBlocked by CG methylation
OriginBacillus stearothermophilus FN
Storage conditions20 mM Tris-HCl (pH 7.5); 300 mM NaCl; 0.1 mM EDTA; 10 mM MgCl2; 200 ug/ml BSA, 7 mM 2-mercaptoethanol and 50% glycerol; Store at -20C.
Assayed onLambda DNA
LigationAfter 5-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)

Enzyme % activity:
BGOWY
50 - 7575 - 10075 - 10050 - 75100