|Catalog number||E549||E550 |
|Package, u.a.||200||1000 |
|Concentration, u.a./ml||5000||5000 |
|Methylation sensitivity||AluB I is able to cleave some methylated DNA substrates.|
|Origin||Arthrobacter luteus B|
|Storage conditions||10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.|
|Assayed on||Lambda DNA|
|Ligation||After 10-fold overdigestion with enzyme 80% of the Lambda DNA fragments can be ligated with T4 DNA Ligase and can be recut.|
|Non-specific activity||No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.|
|Optimum temperature||37 oC|
|Inactivation 20 minutes||65oC|
|Optimum SE-buffer||B (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.) + BSA|
Enzyme % activity:
|100||75 - 100||10 - 25||10 - 25||75 - 100|
Note: AluB I is an isoschizomer of Alu I.
To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
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