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SibEnzyme Ltd. :: Display product information: AluB I
Name
AluB I
Catalog numberE549E550
Package, u.a.2001000
Concentration, u.a./ml50005000

Recognition siteAG^CT
TC^GA
Methylation sensitivityAluB I is able to cleave some methylated DNA substrates.
OriginArthrobacter luteus B
Storage conditions10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20C.
Assayed onLambda DNA
LigationAfter 10-fold overdigestion with enzyme 80% of the Lambda DNA fragments can be ligated with T4 DNA Ligase and can be recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferB (10 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 1 mM DTT.) + BSA

Enzyme % activity:
BGOWY
10075 - 10010 - 2510 - 2575 - 100

Note: AluB I is an isoschizomer of Alu I.
To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.

References:

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