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SibEnzyme Ltd. :: Display product information: BstV2 I
Name
BstV2 I
Catalog numberE297E298
Package, u.a.2001000
Concentration, u.a./ml5000-150005000-15000

Recognition siteGAAGAC(N)2^
CTTCTG(N)6^
Methylation sensitivitynot tested
OriginBacillus stearothermophilus V2
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 200 ug/ml BSA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20C.
Assayed onLambda DNA
LigationAfter 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 5 u.a. of enzyme for 16 hours at 55C.
Optimum temperature55 oC
Inactivation 20 minutes65oC
Optimum SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) + BSA

Enzyme % activity:
BGOWY
75 - 10075 - 10025 - 5025 - 50100

Note: High enzyme concentration may result in star activity.
To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 ug/ml.
Do not use BSA for long incubation.
References: Sinichkina, S.A., Dedkov, V.S., Degtyarev, S.K. Unpublished observations (2000).