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SibEnzyme Ltd. :: Display product information: Bar I
Name
Bar I
Catalog numberE547E548
Package, u.a.100500
Concentration, u.a./ml500-2000500-2000

Recognition site^(N)7GAAGNNNNNNTAC(N)12^
^(N)12CTTCNNNNNNATG(N)7^
Methylation sensitivitynot tested
OriginBacillus sphaericus
Storage conditions20 mM KPO4(pH 7.4); 100 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA, 50% glycerol. Store at -20C.
Assayed onT7 DNA
LigationAfter 2-fold overdigestion with enzyme 90% of DNA fragments can be ligated. Of these 95% can be recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 μg of T7 DNA with 2 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferSE-buffer 2K (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 200 mM KCl; 1 mM DTT.)

Enzyme % activity:
BGOWY
0 - 100 - 1025 - 5050 - 7510 - 25