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SibEnzyme Ltd. :: Display product information: Glu I
Glu I
Catalog numberE519E520
Package, u.a.100500
Concentration, u.a./ml40004000

Recognition site(5mC)G(5mC)^NG(5mC)G
Methylation sensitivityThe enzyme cleaves only methylated DNA.
OriginGlacial ice bacterium GL24
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 0,05% Triton X-100, 50% glycerol; Store at -20C.
Assayed onDouble-stranded oligonucleotide
3' CCATAAG(5mC)  G  (5mC)  G  ACATGAAAGGG 5'
Non-specific activityNo nonspecific activity was detected after incubation of 1 μg of pFsp4HI3 DNA (Bgl II digest) with 4 unit of GluI for 16 hours at 37C. The pFsp4HI3 plasmid carries a double-stranded oligonucleotide 5'- GCCGCGGCAGC -3' / 3'-CGGCGCCGTCG -5' and a gene for M.Fsp4HI DNA-methyltransferase, which modifies DNA forming 5'- G(5mC)CG(5mC)GG(5mC)AGC -3'/3'-CGG(5mC)GC(5mC)GT(5mC)G-5'.
No detectable degradation of a double-stranded labeled oligonucleotide was observed after incubation with 4 unit of restriction endonuclease GluI for 3 hours at 37C.
Optimum temperature37 oC
UnitOne unit is defined as the amount of enzyme required to cleave 1 pmol of the double-stranded oligonucleotide of the above indicated structure in 1 hour at 37C in a total reaction volume of 20 μl.
Inactivation 20 minutes65oC
Optimum SE-bufferSE-buffer GluI (10 mM Tris-HCl (pH 9,0 at 25C); 7,5 mM MgCl2; 75 mM NaCl; 1 mM 2-mercaptoethanol.)

Enzyme % activity:
75 - 10075 - 10075 - 10075 - 10075 - 100


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