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SibEnzyme Ltd. :: Display product information: Bls I
Name
Bls I
Catalog numberE533E534
Package, u.a.100500
Concentration, u.a./ml80008000

Recognition siteG(5mC)N^GC
(5mC)G^N(5mC)G
Methylation sensitivityThe enzyme cleaves only methylated DNA.
OriginBacillus simplex 23
Storage conditions10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg /ml BSA; 50% glycerol; Store at -20C.
Assayed onDouble-stranded oligonucleotide
5 CGTGGTGAGAATCAATG(5mC)CGCTAAAGTACAAGC 3
3' GCACCACTCTTAGTTA(5mC)GG(5mC)GATTTCATGTTCG 5'
Non-specific activityNo nonspecific activity was detected after incubation of 1 μg of pFsp4HI1 DNA (BamHI digest) with 8 unit of BlsI for 16 hours at 30C. The pFsp4HI1 plasmid carries a gene for Fsp4HI DNA-methyltransferase, which modifies DNA forming 5'-G(5mC)NGC-3'/3'-CGN(5mC)G-5'.
Optimum temperature30 oC
UnitOne unit is defined as the amount of enzyme required to cleave 1 pmol of the double-stranded oligonucleotide of the above indicated structure in 1 hour at 30C in a total reaction volume of 20 μl.
Inactivation 20 minutes65oC
Optimum SE-bufferW (10 mM Tris-HCl (pH 8.5 at 25C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)

Enzyme % activity:
BGOWY
10 - 2510 - 2550 - 7510075 - 100


References:

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