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SibEnzyme Ltd. :: Display product information: Set I
Name
Set I
Catalog numberE537E538E538X
Package, u.a.20010001000
Concentration, u.a./ml5000500020000

Recognition siteASST^
^TSSA
DescriptionSet I is a restriction endonuclease with 4 expected recognition sites as well as relaxed non-cognate sites (star sites).
Methylation sensitivitynot tested
OriginE.coli strain that carries the cloned Set I gene from Streptomyces werraensis 37
Storage conditions10 mM Tris-HCl (pH 7.6); 100 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 50% glycerol; Store at -20C.
Assayed onpBR322 DNA
LigationAfter 5-fold overdigestion with enzyme, approximately 50% of the DNA fragments can be ligated with T4 DNA Ligase and recut.
Non-specific activitySet I overnight digestion greatly enhances the star activity. DNA can be digested to small oligos under "star" conditions.
Optimum temperature55 oC
UnitOne unit of the enzyme is the amount required to hydrolyze 1 μg of pBR322 DNA in 1 hour at 55C in a total reaction volume of 50 μl.
Inactivation 20 minutes80oC
Optimum SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)

Enzyme % activity:
BGOWY
25 - 5025 - 5075 - 10075 - 100100