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SibEnzyme Ltd. :: Display product information: BstSN I
Catalog numberE065E066
Package, u.a.2001000
Concentration, u.a./ml5000-100005000-10000

Recognition siteTAC^GTA
Methylation sensitivitynot tested
OriginBacillus stearothermophilus SN
Storage conditions10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 100 ug/ml BSA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at 20C.
Assayed onT7 DNA
LigationAfter 5-fold overdigestion with enzyme about 70% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of T7 DNA with 5 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes80oC
Optimum SE-bufferB (10 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 1 mM DTT.)

Enzyme % activity:
10050 - 750 - 1010 - 2550 - 75

Note: High enzyme concentration results in star activity
References: Myakisheva, T.V., Dedkov, V.S., Kileva, E.V., Abdurashitov, M.A., Shevchenko, A.V., Degtyarev, S.K. Unpublished observations (1995).