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SibEnzyme Ltd. :: Display product information: Rig I
Rig I
Catalog numberE529E530
Package, u.a.100500
Concentration, u.a./ml30003000

Recognition siteGGCCGG^CC
Methylation sensitivitynot tested
OriginRhizobium yangligense
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol; Store at -20C. Storage at -70C is recommended for period longer than 7 days.
Assayed on Adenovirus -2 DNA
LigationAfter 5-fold overdigestion with enzyme > 95% of Ad2 DNA fragments can be ligated with T4 DNA Ligase and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Ad2 DNA with 6 units of enzyme for 16 hours at 37C.
Optimum temperature37 oC
UnitOne unit of the enzyme is the amount required to hydrolyze 1 mkg of Ad2 DNA in 1 hour at 37C in a total reaction volume of 50 mkl.
Inactivation 20 minutes65oC
Optimum SE-bufferSE-buffer RigI (10 mM Tris-HCl (pH 8.5 at 25°C); 5 mM MgCl2; 1mM DTT.) + BSA

Enzyme % activity:
75 - 10050 - 750 - 1010 - 2550 - 75

Note: Do not use BSA for long incubation.
To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 ug/ml.