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SibEnzyme Ltd. :: Display product information: Abs I
Name
Abs I
Catalog numberE535E536
Package, u.a.50250
Concentration, u.a./ml10001000

Recognition siteCC^TCGAGG
GGAGCT^CC
Methylation sensitivitynot tested
OriginArthrobacter species 7M06
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA;0,05% Triton X-100; 50% glycerol; Store at -20C.
Assayed on pUC19SE/DriI - digest
LigationAfter 2-fold overdigestion with enzyme about 90% of the DNA fragments can be ligated with T4 DNA Ligase at 16C and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of DNA with 2 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
UnitOne unit of the enzyme is the amount required to hydrolyze 1 mkg of pUC19SE/DriI -digest in 1 hour at 37C in a total reaction volume of 50 mkl.
Inactivation 20 minutes65oC
Optimum SE-bufferSE-buffer AbsI (10 mM Tris-HCl (pH 9.0 at 25°C); 10 mM MgCl2; 50 mM KCl; 1mM DTT.)

Enzyme % activity:
BGOWY
75 - 10010 - 250 - 1050 - 750 - 10


References:

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