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SibEnzyme Ltd. :: Display product information: PciS I
Name
PciS I
Catalog numberE497E498
Package, u.a.50250
Concentration, u.a./ml500-2000500-2000

Recognition siteGCTCTTC(N)1^
CGAGAAG(N)4^
Methylation sensitivitynot tested
OriginPlanococcus citreus S
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA and 50% glycerol; Store at -20C.
Assayed onLambda DNA
LigationAfter 3-fold overdigestion with enzyme 90% of the DNA fragments can be ligated with T4 DNA Ligase at 16C and 95% of these can be recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 1 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferB (10 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 1 mM DTT.)

Enzyme % activity:
BGOWY
10050 - 750 - 100 - 1075 - 100

References: Chernukhin, V.A., Kileva, E.V., Tomilova, J.E., Dedkov, V.S. Unpublished observations (2006).