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SibEnzyme Ltd. :: Display product information: FaeI
Catalog numberE495E496
Package, u.a.50250
Concentration, u.a./ml500-2000500-2000

Recognition siteCATG^
Methylation sensitivityBlocked by CmATG methylation
OriginFlavobacterium aquatile N3
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol; Store at -20C.
Assayed onpUC19 DNA
LigationAfter 2-fold overdigestion with enzyme more then 90% of the DNA fragments can be ligated with T4 DNA Ligase at 16C and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of pUC19 DNA with 1 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) + BSA

Enzyme % activity:
25 - 5050 - 7510 - 2510 - 25100

Note: To obtain 100% activity, BSA should be added the 1x reaction mix to a final concentration of 100 ug/ml.
Do not use BSA for long incubation.
References: Chernukhin, V.A., Kileva, E.V., Tomilova, J.E., Dedkov, V.S.. Unpublished observations (2006).