|Catalog number||E501||E502 |
|Package, u.a.||200||1000 |
|Concentration, u.a./ml||2000-5000||2000-5000 |
|Methylation sensitivity||Blocked by CG methylation.|
|Origin||Bacillus species AC|
|Storage conditions||10 mM KPO4(pH 7.2); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol. Store at -20°C.|
|Assayed on||Lambda DNA|
|Ligation||After 5-fold overdigestion with enzyme more than 95% of the Lambda DNA fragments can be ligated with T4 DNA Ligase at 16°C and 50% of these can be recut.|
|Non-specific activity||No nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.|
|Optimum temperature||37 oC|
|Inactivation 20 minutes||65oC|
|Optimum SE-buffer||O (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) + BSA|
Enzyme % activity:
|10 - 25||25 - 50||100||75 - 100||10 - 25|
Note: To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 ug/ml.
Do not use BSA for long incubation.
BspACI has a non-palindromic recognition site.
References: Chernukhin, V.A., Tomilova, J.E., Dedkov, V.S., Janobilova, Z.K.. Unpublished observations(2006).