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SibEnzyme Ltd. :: Display product information: BspAC I
Catalog numberE501E502
Package, u.a.2001000
Concentration, u.a./ml2000-50002000-5000

Recognition siteC^CGC
Methylation sensitivityBlocked by CG methylation.
OriginBacillus species AC
Storage conditions10 mM KPO4(pH 7.2); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol. Store at -20C.
Assayed onLambda DNA
LigationAfter 5-fold overdigestion with enzyme more than 95% of the Lambda DNA fragments can be ligated with T4 DNA Ligase at 16C and 50% of these can be recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferO (50 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) + BSA

Enzyme % activity:
10 - 2525 - 5010075 - 10010 - 25

Note: To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 ug/ml.
Do not use BSA for long incubation.
BspACI has a non-palindromic recognition site.
References: Chernukhin, V.A., Tomilova, J.E., Dedkov, V.S., Janobilova, Z.K.. Unpublished observations(2006).